Additional contaminating bacteria will complicate the experiment and possibly confuse the results. Aseptic technique is vital when the effectiveness of antibacterial substances is being tested.

This image highlights the various challenges in maintaining cell culture integrity, including contamination from bacteria, viruses ... and tissue engineering applications. Aseptic technique is a set ...

and that aseptic techniques are used to inoculate the plates. An inoculating loop can be used to transfer bacteria. It is sterilised by heating it to red hot in a Bunsen flame, before and after use.