1. Collect your inoculating loop, as well as your Gram-positive Unknown A and Gram-negative Unknown B, and proceed to the KOH test station. A solution of 3% KOH should be provided there. 2. Place a ...
Collect a bottle of soil culture (nitrogen-free medium). Using an inoculating loop collect a sample of the culture and streak for isolation onto a nitrogen-free agar plate.
An inoculating loop can be used to transfer bacteria. It is sterilised by heating it to red hot in a Bunsen flame, before and after use. To inoculate the agar, lift the lid of the Petri dish and tilt.
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