Pour plate –the original culture is diluted serially and a small volume of the final dilution is added to molten agar which is poured over an agar plate and allowed to harden. Colonies develop ...

Reason - this allows the bacteria to spread out and to grow in individual colonies on the agar plate. A lawn of bacteria ... not been able to reproduce. Soak small (5 mm) filter paper disks ...

Reason – this allows the bacteria to spread out and to grow in individual colonies on the agar plate. A lawn of bacteria can be produced by using a sterile spreader to evenly spread the bacteria ...