Reason – this allows the bacteria to spread out and to grow in individual colonies on the agar plate. A lawn of bacteria can be produced by using a sterile spreader to evenly spread the bacteria ...
KLECZKOWSKA 1 has described a ‘physiological ageing’ of colonies of Rhizobium in an agar layer which renders them immune to attack by bacteriophage after they are 19 hr. old. Thus the ...