Unless stated otherwise, 24 ± 2 h later, the media was changed to mTeSR™1, and all experiments were maintained ... Cell aggregates were dislodged by gently triturating each well with a 10 mL ...
We used a protocol in two steps: (1) induction of hematopoietic differentiation ... 5, 15, and 50 ml sterile centrifuge tubes (Falcon). - 2, 5, 10, 25, and 50 ml sterile serological pipets (Falcon). - ...
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