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Incubate the doubly transformed cells with shaking at 37 °C for 1 h and then plate them on doubly selective LB agar plates (100 μg/ml ampicillin and 35 μg/ml kanamycin). When the transformation ...
The Drosophila Gatewayâ„¢ Vector collection is a set of 68 Gateway-based vectors designed to express epitope-tagged proteins in Drosophila culture cells or flies. At its core is Invitrogen's Gatewayâ„¢ ...
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