(B) Bone cell types (osteoblast, osteocyte, and osteoclasts) - absence or presence of the cell type. (C) Bone formation - formation of connective tissue filling of the defect with blood capillaries, ...

In this study, we report the optimization of our previously developed 3D tissue-engineered in vitro model of human ... μm were made using a cryotome (Thermo fisher Cryostar NX70). The slides were ...

Tissues were then embedded in paraffin. Serial sections (12 µm thick) were cut for haematoxylin and eosin (H and E) and immunohistochemistry (IHC) staining. Immunofluorescence staining was performed ...