eLife1h
Development of a new genotype–phenotype linked antibody screening system
A Golden Gate-based dual-expression vector enables rapid screening, which facilitates efficient isolation of high-affinity cross-reactive antibodies for therapeutic or diagnostic use and provides a ...
eLife14h
Proactive distractor suppression in early visual cortex
This well-written report uses functional neuroimaging in human observers to provide convincing evidence that activity in the early visual cortex is suppressed at locations that are frequently occupied ...
eLife14h
Molecular dynamics simulations illuminate the role of sequence context in the ELF3-PrD-based temperature sensing mechanism in plants
In this potentially valuable computational study, the authors conducted atomistic and coarse-grained simulations to probe the temperature-dependent phase behaviors of ELF3, a disordered component of ...
eLife17h
Multi-species genome-wide CRISPR screens identify conserved suppressors of cold-induced cell death
This study presents important findings on cold tolerance shared between hibernating and non-hibernating mammals, identifying a key molecule, GPX4, through multi-species genome-wide CRISPR screens. The ...
eLife3d
In silico screening by AlphaFold2 program revealed the potential binding partners of nuage-localizing proteins and piRNA-related proteins
(C) Immunoprecipitation of the endogenous Squ from ovarian lysate revealed the interaction with Spn-E protein. Proteins were detected by western blotting analysis using the specific antibody for each ...
eLife3d
Biochemical and structural insights into the auto-inhibited state of Mical1 and its activation by Rab8
The compelling evidence explains how Rab8 family members bind at two sites to allosterically regulate MICAL1, and relieve an auto-inhibited state unable to bind actin. This study lays the basis for ...
eLife3d
Maf-family bZIP transcription factor NRL interacts with RNA-binding proteins and R-loops in retinal photoreceptors
Neurobiology, Neurodegeneration and Repair Laboratory, National Eye Institute, National Institutes of Health, Bethesda, United States This important study employed multiple orthogonal techniques and ...
eLife3d
Introduction of cytosine-5 DNA methylation sensitizes cells to oxidative damage
This important work advances our understanding of DNA methylation and its consequences for susceptibility to DNA damage. This work presents evidence that DNA methylation can accentuate the genomic ...
eLife3d
TET2 contributes to gluconeogenesis and pathology of type 2 diabetes
Department of Radiation Oncology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China ...
eLife4d
Colony demographics shape nest construction in ants
This study presents an important finding that ant nest structure and digging behavior depend on ant age demographics for a ground-dwelling ant species (Camponotus fellah). By asking whether ants ...
eLife5d
Oxytocin salvages context-specific hyperaltruistic preference through moral framing
This study presents a valuable finding that altruistic tendency during moral decision-making is context-dependent (present in the gain domain but absent in the loss domain) and its absence in the loss ...
eLife5d
Astrocytes mediate two forms of spike timing-dependent depression at entorhinal cortex-hippocampal synapses
Revised: This Reviewed Preprint has been revised by the authors in response to the previous round of peer review; the eLife assessment and the public reviews have been updated where necessary by the ...